Abstract: To reveal the potential myocardial and muscle injury of astronauts during long-duration spaceflight, an aptamer-based colloidal gold chromatography Strips were studied to for rapid Creatine Kinase-MB detection. Firstly, the obtained Creatine Kinase-MB aptamer was screened based on a SELEX method. And then a series of aptamer with strong affinity for Creatine Kinase-MB were selected preferably to be applied to the colloidal gold chromatography strips. By optimizing conditions such as the size of colloidal gold particle and the concentration of buffer, a method of aptamer-based lateral flow strips for rapid CK-MB detection was successfully established. The results show that, the aptamers with affinity for Creatine Kinase-MB screened by a magnetic bead-based nucleic acid aptamer screening method can be used to construct an aptamer-based colloidal gold chromatography strips. It has been successfully applied to the rapid and visual detection of Creatine Kinase-MB above 82 nM. At the same time, chromatography strips can be used for future space medical instant detection as its better specificity and stability.