温敏型分子印迹量子点生物传感器用于检测牛血红蛋白

Thermosensitive Molecularly Imprinted Quantum Dots as Biosensor for the Detection of Bovine Hemoglobin

摘要: 制备了一种基于分子印迹量子点的温敏型生物传感器（QD@MIPs），用于牛血红蛋白（BHb）的快速检测.实验结果表明，制备的QD@MIPs对BHb具有快速的荧光响应，吸附平衡时间为15 min.在最优条件下，BHb的荧光猝灭与其浓度成正比，在0.15~2.30 μmol/L的浓度范围内有良好的线性关系，相关系数为0.986 9，检出限为0.097 μmol/L.此外，制备的QD@MIPs具有温敏性质，这一特性使其在生物分析中可以通过改变温度快速吸附和释放模板蛋白.

Abstract: A thermosensitive biosensor based on molecularly imprinted quantum dots (QD@MIPs) was developed and used for rapid detection of bovine hemoglobin (BHb). The experimental results show that, the prepared QD@MIPs can provide a fast fluorescence response to BHb and achieve the adsorption equilibrium in 15 min. Under optimal conditions, the fluorescence quenching of BHb is proportional to the concentration in the range of 0.15~2.3 μmol/L with a correlation coefficient of 0.986 9, and the detection limit is 0.097 μmol/L. Moreover, the prepared QD@MIPs possesses thermosensitive, this feature can endow it rapid adsorption and release of template protein by changing the temperature in bio-analysis.