Abstract: The frequency domain calibration method of pulsed electric field was studied and its uncertainty was evaluated. According to the calibration requirement of pulsed electric field, a frequency domain calibration system based on GTEM cell was established. Through simulation and experiment, the uncertainty of standard electric field produced in GTEM cell and the uncertainty of interaction between sensor and standard electric field were analyzed. Synthesizing the uncertainties, the total uncertainty of the calibration was 4.45% when the confidence probability is more than 95%. Based on this, a three-dimensional pulsed electric field was calibrated in frequency domain. The calibration results show that the sensor has a relatively flat amplitude frequency response in 10 MHz~1 GHz. The research in this paper can provide a certain reference value for analyzing the frequency domain calibration of electric field sensors.