Abstract: Synthesized a novel multifunctional targeting nano liposome drug delivery system,the photothermal sensitive liposome(PTSL),with doxorubicin wrapped in the hydropholic layer as the therapeutical agents and cypate dopped in the hydrophobic layer as the diagnostic agents,and the outer phospholipid modified the targeted transmembrance peptide H<sub<4-1</sub<.The physical properties,stabilities and loading properties of the liposomes were tested,the nano composites we synthesized were targeted,stable and delicate,with a drug loading(DL)of 9±1.5% and encapsulation efficiency(EE)of 82.7±2.1%.The Tm value falls on 43.1℃,the temperature of which can be regarded as the proper temperature which can show the thermal therapy effective as well as not burn the normal tissue.It can raise the temperature of liposome to the Tm of thermal sensitive liposome,the liposome cracks and the wrapped chemotherapy drugs release.At the same time,in the level of organelles,the PTSLs mainly located in the cytoplasm.When exposured to the NIR light,the cypate can incuce the producing of singlet oxygen,causing the crack of the organelle membrane.And the liposome can achieve the functions of target-delivery,enhanced photochemical internalized release,chemotherapy and thremal therapy,indicating that the PTSL is a kind of promising drug delivery system for tumor diagnosis and targeting therapy.