Abstract: The objective of this paper is to extract a comprehensive measure which reflects drivers' microscopic behaviors to measure the safety of urban tunnel entrance and exit. Field test was conducted at Xi'an men tunnel located in Nanjing. The eye-movement and psychophysical performance of seven drivers, together with driving speed and acceleration along the portal and exit of urban tunnel were collected synchronously by the Dikablis. According to weight score of each factor, a comprehensive measure (F_E) was established and it was classified into three levels by the criterion of history traffic crash rate. It could be used to measure the safety of urban tunnel portal and exit zone based on microscopic driving behavior.