Abstract: The uniaxial compressive and tensile tests were performed using an electronic universal testing machine and enhanced Hopkinson technique, over the range of strain rate from 0.001 s<sup<-1</sup< to 5 000 s<sup<-1</sup<, and at temperatures from the room temperature to 1 073 K. Systematic study of the plastic flow behavior and the fracture microstructure characteristics of materials were accomplished. The effects of strain rate hardening and temperature softening can be reflected under compression loading. The formation and expansion of adiabatic shear bands are the primary fracture mechanism under compressive loading. Initial defects are the main reason to induce the formation of shear cracks. The comparison of the 3D laser deposition TC4 and traditional TC4 in mechanical properties shows that the material strength of the former is close to cast TC4 but slightly lower than that of traditional forged TC4 and the plastic performance is slightly superior. The plastic flow behavior of the additive manufactured Ti-6Al-4V can be well described by a physical constitutive model based on the dislocation dynamics and thermal activation mechanism.