Abstract: In order to increase the receiving efficiency of the Lidar wind measurement system, it is necessary to locate the laser spot center with high precision. Based on the analysis of commonly used algorithm, the centroid method was improved by using Gaussian fitting and a rectangular region. Having analyzed and compared the new algorithm with the existing algorithm, finally the averification system was designed and the experiment was carried out. Experimental results indicate that the standard deviation and positioning precision of the new algorithm is both superior to those of Gaussian fitting and centroid method, in addition, the new algorithm can minimize the error caused by asymmetrical spot shape.The comparison results of the accuracy of light energy distribution center show that the new algorithm is also better than Gaussian fitting method and centroid method, which proved the new algorithm is a feasible method for laser spot center location.