Abstract: In the present study, D-GalN/LPS-induced acute liver injury mice model was applied to investigate the effectiveness of hepato-protective and descending transaminase function of BGYH. ICR mice were randomly divided into seven groups: control group, model group, low-, medium- and high-dose BGYH group, silymarin group and bifendate group. All dose groups were orally administrated respective drugs once for one day and continued for 9 days while control and model groups were given equal volume of vehicle. Post the last administration, D-GalN/LPS were injected intraperitoneally into mice except control group. The results show that the levels of ALT and AST in serum are significantly reduced in high-dose BGYH group. Meanwhile, sections of liver tissue also illustrated high-dose BGYH can alleviate necrocytosis of hepatocytes. Moreover, in the mechanism study, significant reduction of TNF-α and IL-6 in serum of medium- and high- dose BGYH group is observed, and concentration of IL-10 is remarkably increased. Furthermore, declining trend of NO and iNOS concentration occures in liver tissue. In conclusion, BGYH possessed protective effects on mice acute liver injury induce by D-GalN/LPS through the immunomodulation pathway.