Thermosensitive Molecularly Imprinted Quantum Dots as Biosensor for the Detection of Bovine Hemoglobin

A thermosensitive biosensor based on molecularly imprinted quantum dots (QD@MIPs) was developed and used for rapid detection of bovine hemoglobin (BHb). The experimental results show that, the prepared QD@MIPs can provide a fast fluorescence response to BHb and achieve the adsorption equilibrium in 15 min. Under optimal conditions, the fluorescence quenching of BHb is proportional to the concentration in the range of 0.15~2.3 μmol/L with a correlation coefficient of 0.986 9, and the detection limit is 0.097 μmol/L. Moreover, the prepared QD@MIPs possesses thermosensitive, this feature can endow it rapid adsorption and release of template protein by changing the temperature in bio-analysis.