New Cell Viability Measurement of SH-SY5Y Cells in the PD Research-Alamarblue^TM

The traditional MTT assay requires destructive analyses and is not convenient in continuous monitory of cell viability. However, a new cell model was developed in this research, by using the oxidation-reduction (redox) indicator alamarblue? instead of the MTT assay. The alamarblue?does no harm to cells and provide a much more safe and convenient methods of measurement. Firstly, cell apoptosis was induced by different concentration of 6-OHDA, then the cell viability was tested by the alamarblue?at a serial of time points. Finally, the optimism cells density, 6-OHDA concentration and testing time point were gained to set a wonderful SH-SY5Y cell model in our research. And when it is applied in the study of neuroprotection effects of NAC, GSH and Catalase, the new model reveals undeniable advantages.